Cell Based Assays
Promega NanoBRET TE Intracellular HDAC Complete Kit, 1,000 assays
NanoBRET Target Engagement Intracellular HDAC Assay measures compound binding at select HDAC target proteins within intact cells. The assay is based on NanoBRET System, an energy transfer technique designed to measure molecular proximity in living cells.
Promega NAD(P)H-Glo™ Detection System
The NAD(P)H-Glo™ Detection System is a bioluminescent assay that measures the concentration of the reduced forms of nicotinamide adenine dinucleotides, NADH and NADPH, and does not discriminate between them.
Promega PYK2 Kinase Enzyme System, 10microg
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega PIM2 Kinase Enzyme System, 1mg
Recombinant full-length human PIM2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PIM2 is a serine threonine kinase that is present in all tissues.
Promega FGFR3 (K650E) Kinase Enzyme System, 1mg
Recombinant human FGFR3 (K650E) (397-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Fibroblast growth factor receptor 3 is part of a family of fibroblast growth factor receptors that share similar structure and function
Promega CDK3/CyclinE1 Kinase Enzyme System, 10microg
Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1 is a member of the cyclin-dependent protein kinase family.
Promega DDR2 Kinase Enzyme System, 10microg
Recombinant human DDR2 (467-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. DDR2 is a member of a novel subclass of RTKs containing a distinct extracellular region encompassing a factor VIII-like domain.
Promega ASK1 Kinase Enzyme System, 10microg
Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death.
Promega CDK5/p25 Kinase Enzyme System, 1mg
Recombinant full-length human CDK5 and p25 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.
Promega DYRK2 Kinase Enzyme System, 10microg
Recombinant full-length human DYRK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega NUAK2 Kinase Enzyme System, 10microg
Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2.
Promega ULK1 Kinase Enzyme System, 1mg
Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ULK1 is a serine/threonine protein kinase that plays a critical role during initial stages of autophagy, a vital response to nutrient starvation.
Promega PI3K (p120gamma), 20ug, 200microl
The PI3K lipid kinase enzymes are used with ADP-Glo KInase Assay System and Lipid Kinase Substrates in a homogeneous, add-mix-read lipid kinase assay.
Promega ERK1 Kinase Enzyme System, 10microg
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega TNIK Kinase Enzyme System, 10microg
Recombinant human TNIK (1-367) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TNIK or TRAF2 and NCK-interacting kinase are characterized by N-terminal kinase and C-terminal GCK domains that serve a regulatory function.